Figure 1. Generation of Snap23 KO mice and expression of SNAP23 and SNAP25 in the pancreas. (A) Restriction maps of the wild-type allele, targeting vector, targeted allele, floxed allele, and null allele. Arrowheads indicate the position of the primers used for PCR screening. (B) Genotypic distribution of wild-type (WT; Snap23+/+), heterozygous (HET; Snap23+/− and Snap23+/geo), and KO (Snap23−/− and Snap23geo/geo) mice at postnatal day (P) 7 and embryonic days (E) 10.5, 9.5, and 8.5, raised by intercrossing heterozygous pairs. No homozygous mutant SNAP23 mice (KO) were obtained. (C and D) In the wild-type pancreas, SNAP23 is localized in both the islets and acini, but SNAP25 is localized only in the islets. (C) Expression patterns of SNAP23 and SNAP25 in wild-type mouse pancreas shown by immunohistochemistry. High-magnification figures are shown in the top right (islet) and bottom right (acini). The SNAP25 antibody nonspecifically stained extracellular matrix surrounding lobules of acini (arrow). Bars, 20 µm. (D) Expression patterns of SNAP23 and SNAP25 in the human pancreas shown by immunohistochemistry. Regions surrounded by dashed lines indicate the pancreatic islets. Bar, 50 µm. IRES, internal ribosomal entry site; SA, splice acceptor.
Figure 1.

Generation of Snap23 KO mice and expression of SNAP23 and SNAP25 in the pancreas. (A) Restriction maps of the wild-type allele, targeting vector, targeted allele, floxed allele, and null allele. Arrowheads indicate the position of the primers used for PCR screening. (B) Genotypic distribution of wild-type (WT; Snap23+/+), heterozygous (HET; Snap23+/− and Snap23+/geo), and KO (Snap23−/− and Snap23geo/geo) mice at postnatal day (P) 7 and embryonic days (E) 10.5, 9.5, and 8.5, raised by intercrossing heterozygous pairs. No homozygous mutant SNAP23 mice (KO) were obtained. (C and D) In the wild-type pancreas, SNAP23 is localized in both the islets and acini, but SNAP25 is localized only in the islets. (C) Expression patterns of SNAP23 and SNAP25 in wild-type mouse pancreas shown by immunohistochemistry. High-magnification figures are shown in the top right (islet) and bottom right (acini). The SNAP25 antibody nonspecifically stained extracellular matrix surrounding lobules of acini (arrow). Bars, 20 µm. (D) Expression patterns of SNAP23 and SNAP25 in the human pancreas shown by immunohistochemistry. Regions surrounded by dashed lines indicate the pancreatic islets. Bar, 50 µm. IRES, internal ribosomal entry site; SA, splice acceptor.

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