Proximal signaling and cytokine secretion upon FcεRI activation is not affected in Kif5b-deficient BMMCs. (A) IgE-sensitized WT and cKO^Kif5b BMMCs were stimulated with DNP-HSA for the indicated times, lysed, and then assayed for total and phosphorylated Akt and Erk by Western blotting. Data are representative of two independent experiments. (B) IgE-sensitized WT and cKO^Kif5b BMMCs were loaded with indo-1 and stimulated with DNP-HSA. The fluorescence emission ratio for Ca²⁺-bound/Ca²⁺-free indo-1 was measured for the indicated period of time. Similar results were obtained in three independent experiments. (C) TNF, IL-6, and CCL2 (MCP1) secretion were determined in ELISA. Data are expressed as the mean ± SD of triplicate samples and are representative of two independent experiments. ns, not significant.