TRPA1 is a lysosomal Ca²⁺ release channel in DRG neurons. Several TRP channels are localized at both plasma membranes and intracellular membranes, such as ER, secretory vesicles, endosomes, and lysosomes. The compositions of lipids (PI(3,5)P₂ for lysosomes and PI(4,5)P₂ for the plasma membrane) and luminal ions are indicated for each compartment. In the DRG neurons, TRPA1 has a dual location: plasma membrane (PI(4,5)P₂ enriched) and lysosomes. Upon activation by TRPA1 agonists such as AITC, plasma membrane TRPA1 mediates Ca²⁺ influx and lysosomal TRPA1 mediates Ca²⁺ release from lysosomes ([Ca²⁺]_lumen is ∼0.5 mM) at the periphery of the cell near plasma membrane. Ca²⁺ release through TRPA1 activation is sufficient to activate Ca²⁺ sensors (e.g., synaptotagmins) in the VAMP2-localized DCVs, triggering DCV exocytosis and neuropeptide (CGRP and NPY) release. TRPMLs are present on all lysosomes (pH 4.6; [Ca²⁺]_lumen is ∼0.5 mM Ca²⁺; PI(3,5)P₂), mediating Ca²⁺-dependent lysosomal exocytosis, membrane trafficking, and transport through Ca²⁺ sensors (calcineurin/ALG-2/CaM/synaptotagmins). In the cytoplasm, the ER may provide Ca²⁺ to lysosomes through ER–lysosome membrane contact sites.