Figure 2. Mitochondria regulate calcineurin activity in response to TORC2-Ypk1. (A) Cells carrying pAMS363 that expressed 2xCDRE:lacZ were grown to log phase in SCD medium without uracil. β-Galactosidase (β-gal) activity was measured and is displayed as units of nmol ONPG converted/min/mg of protein (see Materials and methods). Data are presented as means ± SD of three independent experiments. (B) Strains were grown to log phase as in A and incubated with 10 µM DCF for 30 min before fluorescence microscopy imaging. Quantification represents the percentage of DCF-positive fluorescing cells where n ≥ 300 total cells per strain. Data are presented as means ± SD of three independent experiments. (C) WT and ypk1Δ cells carrying pAMS363 were grown as in A in the presence and absence of 20 mM NAC, and β-galactosidase activity was measured. Data are presented as means ± SD of three independent experiments.
Figure 2.

Mitochondria regulate calcineurin activity in response to TORC2-Ypk1. (A) Cells carrying pAMS363 that expressed 2xCDRE:lacZ were grown to log phase in SCD medium without uracil. β-Galactosidase (β-gal) activity was measured and is displayed as units of nmol ONPG converted/min/mg of protein (see Materials and methods). Data are presented as means ± SD of three independent experiments. (B) Strains were grown to log phase as in A and incubated with 10 µM DCF for 30 min before fluorescence microscopy imaging. Quantification represents the percentage of DCF-positive fluorescing cells where n ≥ 300 total cells per strain. Data are presented as means ± SD of three independent experiments. (C) WT and ypk1Δ cells carrying pAMS363 were grown as in A in the presence and absence of 20 mM NAC, and β-galactosidase activity was measured. Data are presented as means ± SD of three independent experiments.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal