TPCL is formed from CL of condensed mitochondria. (a) Subcellular fractions were prepared from testis homogenate as shown in Fig. 2 a, and CL synthase activity was measured. (b) Orthodox and condensed mitochondria were separated by Percoll density gradient centrifugation and affinity-purified with the Tom22 antibody. LNMs were recovered from the anti-Tom22 flow through of condensed mitochondria. CL was analyzed by LC-MS/MS. Bar graphs show mean values ± SEMs (n = 3). (c and d) CL and monolyso-CL (MLCL) from whole testes were analyzed by LC-MS/MS. Bar graphs show mean values ± SEMs (n = 3). (e) Histological sections showing the seminiferous tubules of mouse testes were stained with hematoxylin and eosin. (f) The distribution of germ cell types was determined in electron micrographs from mouse testes. Bar graphs show mean values ± SEMs (n = 3). Wild-type and TAZ KO were compared by the χ2 test. (g) Electron micrographs show different categories of mitochondria in normal male germ cells. (h) Orthodox, intermediate, and condensed mitochondria were quantified in different germ cells of mouse testes by using EM. The total number of mitochondria analyzed in each cell type is given on top. The composition of mitochondria was different between wild type and TAZ-KO in each cell type (P < 0.0001, χ2 test).
