Figure 4.
Figure 4. Expression of Sgo1 ΔSET or SET depletion delays chromosome segregation. (A) Time-lapse analysis of RPE-1 cells expressing H2B-GFP and transfected with Mock, Separase (Sep), or SET siRNAs. (B) Quantification of the duration from metaphase to chromosome segregation of mitotic cells in A. The average and standard deviation are shown here. Mock, n = 50 cells; siSep, n = 50 cells; siSET, n = 50 cells. ****, P < 0.0001. One-way ANOVA was performed followed by pairwise comparisons using Tukey’s test. (C) Time-lapse analysis of H2B-GFP RPE-1 cells stably expressing exogenous Sgo1 WT or ΔSET transfected with luciferase (Mock) or Sgo1 siRNAs. (D) Quantification of the duration from metaphase to chromosome segregation in mitotic cells in C. The average and standard deviation are shown here. Mock, n = 45 cells; WT, n = 41 cells; ΔSET, n = 51 cells. ****, P < 0.0001. One-way ANOVA was performed followed by pairwise comparisons using Tukey’s test.

Expression of Sgo1 ΔSET or SET depletion delays chromosome segregation. (A) Time-lapse analysis of RPE-1 cells expressing H2B-GFP and transfected with Mock, Separase (Sep), or SET siRNAs. (B) Quantification of the duration from metaphase to chromosome segregation of mitotic cells in A. The average and standard deviation are shown here. Mock, n = 50 cells; siSep, n = 50 cells; siSET, n = 50 cells. ****, P < 0.0001. One-way ANOVA was performed followed by pairwise comparisons using Tukey’s test. (C) Time-lapse analysis of H2B-GFP RPE-1 cells stably expressing exogenous Sgo1 WT or ΔSET transfected with luciferase (Mock) or Sgo1 siRNAs. (D) Quantification of the duration from metaphase to chromosome segregation in mitotic cells in C. The average and standard deviation are shown here. Mock, n = 45 cells; WT, n = 41 cells; ΔSET, n = 51 cells. ****, P < 0.0001. One-way ANOVA was performed followed by pairwise comparisons using Tukey’s test.

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