Actinonin induces a time-dependent aberrant accumulation of mitochondrial polypeptides in the inner membrane. (A, left) Pulse 35S metabolic labeling with ethanol (Etoh) or actinonin (Act) in MEFs for the indicated time points. The asterisks indicate the accumulation of novel translation products. (right) Signal quantification determined for all translation products relative to the signal intensity at 30 min in the control from two independent experiments. (B) Quantification of 35S signal intensity with time of selected mitochondrial polypeptides. (C, top) Sodium carbonate extraction of 35S-labeled mitochondrial translation products. (bottom) Immunoblotting for membrane-anchored mt-Co1 and ribosomal protein Mrpl13. a.u., arbitrary units.
