Adherens junctions are enhanced in total intensity and density during the apical shift. (See also Fig. S2.) (A) Projections of a single cell visualized by E-Cad::GFP show the change in intensity and density of the junctions during gastrulation. Bar, 2 µm. (B and C) Changes in the mean intensity of E-Cad signal at individual edge (B) and vertex (C) junctions (averaged by the number of nonzero pixels after image thresholding). (D and E) Changes in the total amount of E-Cad at individual edge (D) and vertex (E) junctions. For B–E, the values at different time points of each edge or vertex sample are normalized by the maximum value of that edge or vertex during the time period presented. (F) A FRAP region compared with an unbleached control region in the same embryo. Bar, 10 µm. (G) The percentage of bleached molecules recovered from the beginning of junction shift, calculated by comparing fluorescence intensity in the control and bleached regions. FRAP was performed on three embryos for both dorsal and ventral regions.