CLAMP controls PCP protein asymmetry. (A–G) GFP-Pk2 (green) asymmetrically accumulates on the posterior side of cells (A and C) which we quantify (n > 50 cells for each condition) by measuring the fluorescent intensity on the posterior side versus the anterior side (E–G) of control (CNTL) cells (relative to mem-RFP, red, in F). This asymmetry is lost in CLAMP morphant tissues marked with mem-RFP (red) both cell-autonomously (A, B, and F) and non–cell-autonomously (C, D, and G). (H–J) Dvl1-GFP (green) asymmetrically accumulates on the anterior side of cells (H and J), which we quantify by measuring the fluorescent intensity on the posterior side versus the anterior side (J) of the cell relative to mem-RFP (red) in control MO (n = 82 cells) and CLAMP MO (n = 36 cells). Graphs are whisker plots where the error bars represent the range; the line in the box represents the median and the box represents the upper and lower quartile. Posterior is to the right. Bars, 5 µm.