Figure 2. CLAMP affects cilia and MCC polarity. (A and B) Cilia polarity as assessed by the position of the rootlet marked with Mig12-GFP (green) relative to basal body marked with centrin4-RFP (red) in control MO (A) and CLAMP MO 5 ng (B) cells. Insets (A′ and B′) show the orientation of individual cilia. In all pictures and in all the quantification throughout the paper, anterior is to the left and posterior is to the right. (C) Quantification of morphant MCC polarity (n = 46 cells for each condition) as scored by the mean cilia direction within each cell (arrow direction) and the variation around that mean (arrow length such that long arrow represents low variance). (D–F) Similar analysis to A–C done on ectopic MCCs induced by the expression of MCIDAS using mosaic tissue injected with 10 ng of MO (n = 61 cells for control MO and 82 for CLAMP MO). Posterior is to the right. Bars: 5 µm; (insets) 2.5 µm.
Figure 2.

CLAMP affects cilia and MCC polarity. (A and B) Cilia polarity as assessed by the position of the rootlet marked with Mig12-GFP (green) relative to basal body marked with centrin4-RFP (red) in control MO (A) and CLAMP MO 5 ng (B) cells. Insets (A′ and B′) show the orientation of individual cilia. In all pictures and in all the quantification throughout the paper, anterior is to the left and posterior is to the right. (C) Quantification of morphant MCC polarity (n = 46 cells for each condition) as scored by the mean cilia direction within each cell (arrow direction) and the variation around that mean (arrow length such that long arrow represents low variance). (D–F) Similar analysis to A–C done on ectopic MCCs induced by the expression of MCIDAS using mosaic tissue injected with 10 ng of MO (n = 61 cells for control MO and 82 for CLAMP MO). Posterior is to the right. Bars: 5 µm; (insets) 2.5 µm.

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