Oma1 deletion in Phb2^NKO mice prevents neuroinflammation. (A) Reactive astrogliosis and activation of microglia in Phb2^NKO mice is blocked by deletion of Oma1. Immunohistochemical staining of coronal sections across the hippocampal region from 14-wk-old mice using GFAP- and IBA1-specific antibodies. Bars, 200 µm. (B) Immunoblot analysis of hippocampal lysates from 14-wk-old mice. GAPDH was used as a loading control. (C) mRNA levels of proinflammatory cytokines in Phb2^NKO mice are restored upon deletion of Oma1. Total RNA was isolated from the hippocampus of 14-wk-old mice and subjected to quantitative real-time PCR analysis (n = 3). Transcript levels were normalized to Gapdh mRNA levels. **, P < 0.01. Error bars indicate SD.