Mesenchymal pancreatic cancer cells assume epithelial phenotype after SUV420H2 knockdown. (A) Bright field microscope images of PANC-1 cells grown in monolayer after NTC control or SUV420H2 knockdown. Yellow dashed lines demarcate boundaries of compact cell groups in cells treated with siSUV420H2. Bars, 50 µm. (B) Migration assay of mesenchymal pancreatic cancer cell lines with NTC control or SUV420H2 knockdown. Representative images of PANC-1 cells are shown. Cells growing in monolayer underwent two rounds of siRNA transfection at days 1 and 4 of the assay. Cells reached confluence at day 5, when a scratch wound was made, and relative wound density was recorded over the next 30 h (Hs 766T and KP4) or 36 h (PANC-1). n = 6 replicates, data depicted as mean ± SD. Differences were assessed by Student’s t test compared with siNTC control. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bars, 50 µm. (C) Histograms depicting data from invasion assay of mesenchymal pancreatic cancer cells with NTC control or SUV420H2 knockdown. Cells growing in monolayer underwent two rounds of siRNA transfection at days 1 and 4 of the assay and were transferred at day 5 to Matrigel-coated transwell plates, and invasion was measured 30 h (Hs 766T and KP4) or 36 h (PANC-1) later. Data are normalized for total number of cells in each well. n = 6 independent experiments. Data depicted as mean ± SD. Differences were assessed by Student’s t test compared with siNTC control. *, P < 0.05; **, P < 0.01; ***, P < 0.001.