Figure 5.
![Figure 5. Thrombin-activated PAR1 induces p38 autophosphorylation. (A and B) PAR1-expressing HeLa cells (A) and endothelial cells (B) were pretreated with 5 µM SB203580 for 20 min and then stimulated with 10 nM α-Th, and phosphorylation of p38, MSK1, ERK1/2, MKK3, and MKK6 was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). See Fig. S3 (A and B). (C) PAR1 HeLa cells were pretreated with SB203580 as described above and then incubated with 10 nM α-Th and 400 µM NaCl, and phosphorylation of p38, MKK3, and MKK6 was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (**, P < 0.01).](https://cdn.rupress.org/rup/content_public/journal/jcb/210/7/10.1083_jcb.201504007/6/jcb_201504007_fig5.jpeg?Expires=1767715486&Signature=f~fJ1QDQpflJPnGKc~4Q1vU9xVQid4bnG8d~dnIg0NF5gCCDY5hGm5y-UZSu7Jm49kSpOloxW1eRLJJwDECzmLDJ11P~IekvCLpZGNTDgbfoZ1hLMl9Snm9EiWT6J1cw89TmWOIMsUcTLnHcxK5oTxWSAIDBN28DQGZi~mebm1EvPtLOVfGBqF8MDYh9kPnG951~II5ZAlRtK5JFFU2WBflusg~hhVCOFo48m2nOEF707W7lo9UHk-tYjoWVzTETHN1q9-QJAuKgR7Uh3tiea7TpmgaclnI~cgawBSI1df02xdY8CuPiKGWnfXlkNBSnoAf1CTmgHXOhASqvEpOWtA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Thrombin-activated PAR1 induces p38 autophosphorylation. (A and B) PAR1-expressing HeLa cells (A) and endothelial cells (B) were pretreated with 5 µM SB203580 for 20 min and then stimulated with 10 nM α-Th, and phosphorylation of p38, MSK1, ERK1/2, MKK3, and MKK6 was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). See Fig. S3 (A and B). (C) PAR1 HeLa cells were pretreated with SB203580 as described above and then incubated with 10 nM α-Th and 400 µM NaCl, and phosphorylation of p38, MKK3, and MKK6 was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (**, P < 0.01).
