Figure 5. The Dsc complex and Cdc48 are important for Cot1-GFP degradation. (A) Localization of tet-O7 Cot1-GFP after Zn2+ withdrawal in WT, deletion mutants for the Dsc complex and the cdc48E315K allele. (B) Degradation kinetics for tet-O7 Cot1-GFP in WT, dsc2Δ, dsc3Δ, and ubx3Δ mutant strains. G6PDH was used as a loading control. Same volume of cells was loaded, with 1 OD600 cells loaded at 0 h. (C) Degradation kinetics for tet-O7 Cot1-GFP in WT and cdc48E315K mutant strains. G6PDH was used as a loading control. Same volume of cells was loaded; with 1 OD600 cells loaded at 0 h. (D) Localization of chromosomally tagged Cot1-GFP before and after Zn2+ withdrawal in dsc deletion mutants overexpressing Tul1. (E) Degradation kinetics of chromosomally tagged Cot1-GFP in dsc deletion mutants overexpressing Tul1. G6PDH was used as a loading control. 1 OD600 of cells were loaded in each lane. Bar, 1 µm.
Figure 5.

The Dsc complex and Cdc48 are important for Cot1-GFP degradation. (A) Localization of tet-O7 Cot1-GFP after Zn2+ withdrawal in WT, deletion mutants for the Dsc complex and the cdc48E315K allele. (B) Degradation kinetics for tet-O7 Cot1-GFP in WT, dsc2Δ, dsc3Δ, and ubx3Δ mutant strains. G6PDH was used as a loading control. Same volume of cells was loaded, with 1 OD600 cells loaded at 0 h. (C) Degradation kinetics for tet-O7 Cot1-GFP in WT and cdc48E315K mutant strains. G6PDH was used as a loading control. Same volume of cells was loaded; with 1 OD600 cells loaded at 0 h. (D) Localization of chromosomally tagged Cot1-GFP before and after Zn2+ withdrawal in dsc deletion mutants overexpressing Tul1. (E) Degradation kinetics of chromosomally tagged Cot1-GFP in dsc deletion mutants overexpressing Tul1. G6PDH was used as a loading control. 1 OD600 of cells were loaded in each lane. Bar, 1 µm.

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