Figure 6.
Figure 6. Knockdown of Bnip3Δex3 promotes cell death induced by doxorubicin. (A) Cell viability of neonatal cardiac myocytes (NCMC), Panc-1, and HCT116 cells treated with different concentrations of doxorubicin for 18 h. Representative epifluorescence images of cells stained with vital dyes calcein AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively, are shown. Bars, 40 µm. (B) Histogram for quantitative data shown in A. Data were obtained from at least n = 3–4 independent experiments counting ≥500 cells from n = 3 glass coverslips for each condition tested. (C) ROS production by DHE staining in Panc-1 cells treated with DOX (10 µM) for 18 h in the absence or presence of Bnip3Δex3 knockdown with siRNA-Bnip3Δex3. Bars, 40 µm. (D) Cell viability of Panc-1 and HCT-116 cancer cells treated with doxorubicin (10 µM) for 18 h in the absence and presence of Bnip3Δex3 knockdown with siRNA-Bnip3Δex3. Representative epifluorescence images of cells stained with vital dyes calcein AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively, are shown. Bars, 40 µm. (E) Histogram for quantitative data shown in D. Data were obtained from at least n = 3–4 independent experiments counting ≥500 cells from n = 3 glass coverslips for each condition tested. (F) Cell viability of cancer cells treated with doxorubicin (10 µM) for 18 h in the absence or presence of siRNA-Bnip3Δex3 and shRNA-Bnip3FL, respectively. Representative epifluorescence images of cells stained with vital dyes calcein AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively, are shown. Bars, 40 µm. (G) Histogram for quantitative data shown in F. Data were obtained from at least n = 3–4 independent experiments counting ≥500 cells from n = 3 glass coverslips for each condition tested. Data are expressed as mean ± SD (error bars). *, statistically different from control (CTRL).

Knockdown of Bnip3Δex3 promotes cell death induced by doxorubicin. (A) Cell viability of neonatal cardiac myocytes (NCMC), Panc-1, and HCT116 cells treated with different concentrations of doxorubicin for 18 h. Representative epifluorescence images of cells stained with vital dyes calcein AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively, are shown. Bars, 40 µm. (B) Histogram for quantitative data shown in A. Data were obtained from at least n = 3–4 independent experiments counting ≥500 cells from n = 3 glass coverslips for each condition tested. (C) ROS production by DHE staining in Panc-1 cells treated with DOX (10 µM) for 18 h in the absence or presence of Bnip3Δex3 knockdown with siRNA-Bnip3Δex3. Bars, 40 µm. (D) Cell viability of Panc-1 and HCT-116 cancer cells treated with doxorubicin (10 µM) for 18 h in the absence and presence of Bnip3Δex3 knockdown with siRNA-Bnip3Δex3. Representative epifluorescence images of cells stained with vital dyes calcein AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively, are shown. Bars, 40 µm. (E) Histogram for quantitative data shown in D. Data were obtained from at least n = 3–4 independent experiments counting ≥500 cells from n = 3 glass coverslips for each condition tested. (F) Cell viability of cancer cells treated with doxorubicin (10 µM) for 18 h in the absence or presence of siRNA-Bnip3Δex3 and shRNA-Bnip3FL, respectively. Representative epifluorescence images of cells stained with vital dyes calcein AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively, are shown. Bars, 40 µm. (G) Histogram for quantitative data shown in F. Data were obtained from at least n = 3–4 independent experiments counting ≥500 cells from n = 3 glass coverslips for each condition tested. Data are expressed as mean ± SD (error bars). *, statistically different from control (CTRL).

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