Figure 4.
Figure 4. Knockdown of Bnip3Δex3 sensitizes cancer cells to Bnip3FL-induced cell death. (A) Cell viability of cancer cells expressing Bnip3FL in the absence and presence of siRNA-Bnip3Δex3. Representative epifluorescence images of various cancer cells stained with vital dyes calcein-AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively. Bars, 40 µm. (B) Histogram for quantitative data shown in A. Data were obtained from at least n = 3–4 independent cell cultures counting ≥500 cells from n = 3 glass coverslips for each condition tested. *, statistically different from control (CTRL). (C) ROS production in Panc-1 cells (red fluorescence) overexpressing Bnip3FL in the absence and presence of Bnip3Δex3 knockdown with siRNA-Bnip3Δex3. Bars, 40 µm.

Knockdown of Bnip3Δex3 sensitizes cancer cells to Bnip3FL-induced cell death. (A) Cell viability of cancer cells expressing Bnip3FL in the absence and presence of siRNA-Bnip3Δex3. Representative epifluorescence images of various cancer cells stained with vital dyes calcein-AM and ethidium homodimer-1 to visualize live (green) and dead (red) cells, respectively. Bars, 40 µm. (B) Histogram for quantitative data shown in A. Data were obtained from at least n = 3–4 independent cell cultures counting ≥500 cells from n = 3 glass coverslips for each condition tested. *, statistically different from control (CTRL). (C) ROS production in Panc-1 cells (red fluorescence) overexpressing Bnip3FL in the absence and presence of Bnip3Δex3 knockdown with siRNA-Bnip3Δex3. Bars, 40 µm.

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