Replicative lifespan of myo2 mutants. (A–C) Replicative lifespan (RLS) of WT (n = 87), Δsir2 (n = 45), and myo2(LQ) (n = 163) was analyzed in three independent experiments with at least 12 cells per strain in each experiment. Mean values are shown in A. A RLS analysis representing the pooled values of the same experiments is shown in B. In C, cells were grouped into the following categories: very short lived (0–4 divisions), short-lived (5–14 divisions), normal (15–29 divisions), long-lived (30–39 divisions), and very long-lived (40–70 divisions). (D–F) Inheritance of vacuoles and mitochondria was analyzed in Δmyo2 cells containing plasmids expressing MYO2, myo2(LQ), or myo2(D1297N) from the MYO2 promoter. (G) RLS analysis was performed as in B; MYO2 (n = 104), myo2(LQ) (n = 180), myo2(D1297N) (n = 131). (H and I) Inheritance of mitochondria was analyzed in cells containing either an empty vector (ctrl) or overexpressing YPT11 from the GPD promoter. Bar, 5 µm. (J) Strains were grown on selective media plates, individual cells were arrayed on a grid, mother cells were removed after the first division, and juvenile cells were scored for their ability to produce colonies. Bars, 5 µm. Data pooling and statistics are detailed in Table S4.