Figure 4. Mitochondrial inheritance defects of fragmented mitochondria. (A and B) Cells were analyzed as in Fig. 3 (B–D) at 30°C. Bar, 5 µm. (C) Δfzo1 cells expressing mtGFP and Δfzo1 Δdnm1 cells expressing mtERFP from galactose-inducible promoters were grown overnight in galactose-containing selective complete medium supplemented with 1% raffinose, washed in glucose-containing rich medium, and equal amounts of cells mixed. Cells were allowed to mate for 5 h in glucose-containing rich medium, fixed with formaldehyde, and mitochondrial inheritance to medial buds analyzed. A representative image shows a zygote inheriting mitochondria only from the Δfzo1 Δdnm1 parent. The asterisk indicates the bud. Bar, 5 µm. Data pooling and statistics are detailed in Table S4.
Figure 4.

Mitochondrial inheritance defects of fragmented mitochondria. (A and B) Cells were analyzed as in Fig. 3 (B–D) at 30°C. Bar, 5 µm. (C) Δfzo1 cells expressing mtGFP and Δfzo1 Δdnm1 cells expressing mtERFP from galactose-inducible promoters were grown overnight in galactose-containing selective complete medium supplemented with 1% raffinose, washed in glucose-containing rich medium, and equal amounts of cells mixed. Cells were allowed to mate for 5 h in glucose-containing rich medium, fixed with formaldehyde, and mitochondrial inheritance to medial buds analyzed. A representative image shows a zygote inheriting mitochondria only from the Δfzo1 Δdnm1 parent. The asterisk indicates the bud. Bar, 5 µm. Data pooling and statistics are detailed in Table S4.

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