Defective mitochondrial inheritance in transport and fusion mutants. (A) 10-fold serial dilutions of cell suspensions were spotted on glucose-containing rich medium and incubated at the indicated temperatures. (B–D) Cells expressing mtGFP were grown at 25°C, cell walls were stained with calcofluor, cultures were split and incubated at the indicated temperatures for 1.5 h, and mitochondrial inheritance was scored. Bar, 5 µm. Data pooling and statistics are detailed in Table S4.