Rescue of mitochondrial inheritance defects by deletion of the DNM1 gene. (A) 10-fold serial dilutions of cell suspensions were spotted on glucose-containing rich medium and incubated at the indicated temperatures. (B) Cells expressing mtGFP were analyzed by fluorescence microscopy. Arrows indicate inherited mitochondria in myo2(LQ) Δdnm1 cells. Bar, 5 µm. (C) Cells containing (ρ⁺, black bars) or lacking mtDNA (ρ⁰, gray bars) were analyzed as in B, and buds were scored for the presence of mitochondria. Significance was calculated in comparison to myo2(LQ) both for ρ⁺ and ρ⁰ cells. The Δfzo1 mutant is always ρ⁰ and was analyzed in a separate experiment and compared with WT. Data pooling and statistics are detailed in Table S4.