Figure 1. Genetic interactions of myo2(LQ). (A) Yeast cells expressing mtGFP were analyzed by fluorescence microscopy. Representative images are DIC merged with maximum intensity projections of z stacks. (B) Negative genetic interactors of myo2(LQ) were analyzed for GO term enrichments in the category “process.” (C) Tetrads of heterozygous myo2(LQ) Δnum1 cells were dissected on glucose-containing rich medium. (D) The colony size was determined after tetrad dissection and normalized to WT (fitness). The red line marks the expected fitness of the double mutant (i.e., the product of the two single mutants’ fitness). (E and F) Cells expressing mtGFP were analyzed by fluorescence microscopy. (G) Cells were fixed, stained with DAPI, and analyzed by fluorescence microscopy. Arrows indicate cells with supernumerary nuclei. The percentage of cells with supernumerary nuclei was scored in at least 100 cells per strain (triplicate experiments ± SD). (H) Strains were crossed as indicated, sporulated, and tetrads were dissected on glucose-containing rich medium. (I) 10-fold serial dilutions of cell suspensions were spotted on glucose-containing rich medium and incubated at 30°C. Bars, 5 µm. Data pooling and statistics are detailed in Table S4.
Figure 1.

Genetic interactions of myo2(LQ). (A) Yeast cells expressing mtGFP were analyzed by fluorescence microscopy. Representative images are DIC merged with maximum intensity projections of z stacks. (B) Negative genetic interactors of myo2(LQ) were analyzed for GO term enrichments in the category “process.” (C) Tetrads of heterozygous myo2(LQ) Δnum1 cells were dissected on glucose-containing rich medium. (D) The colony size was determined after tetrad dissection and normalized to WT (fitness). The red line marks the expected fitness of the double mutant (i.e., the product of the two single mutants’ fitness). (E and F) Cells expressing mtGFP were analyzed by fluorescence microscopy. (G) Cells were fixed, stained with DAPI, and analyzed by fluorescence microscopy. Arrows indicate cells with supernumerary nuclei. The percentage of cells with supernumerary nuclei was scored in at least 100 cells per strain (triplicate experiments ± SD). (H) Strains were crossed as indicated, sporulated, and tetrads were dissected on glucose-containing rich medium. (I) 10-fold serial dilutions of cell suspensions were spotted on glucose-containing rich medium and incubated at 30°C. Bars, 5 µm. Data pooling and statistics are detailed in Table S4.

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