Figure 7. DHPG stimulates proteasome activity and GluA1 ubiquitination. (A) Hippocampal slices were treated with DHPG in the absence or presence of MG132 for the indicated times and samples were homogenized and processed for Western blotting in 16% tricine gel. Data are presented as the ratio (fold of control) of free ubiquitin (Ub) over 14-3-3γ. *, P < 0.05 versus control (n = 7–10, one-way ANOVA). Error bars indicate means ± SEM. (B) Hippocampal slices were incubated with DMSO or G1+DHPG (G1+D) in the presence of MG132. Samples were then analyzed by Western blotting before (input) or after immunoprecipitation (IP) with GluA1 antibody or IgG. The right panel shows an immunoblot for GluA1 and a merged image of immunoblots for GluA1 (red) and ubiquitin (green). Asterisks indicate bands corresponding to ubiquitinated GluA1.
Figure 7.

DHPG stimulates proteasome activity and GluA1 ubiquitination. (A) Hippocampal slices were treated with DHPG in the absence or presence of MG132 for the indicated times and samples were homogenized and processed for Western blotting in 16% tricine gel. Data are presented as the ratio (fold of control) of free ubiquitin (Ub) over 14-3-3γ. *, P < 0.05 versus control (n = 7–10, one-way ANOVA). Error bars indicate means ± SEM. (B) Hippocampal slices were incubated with DMSO or G1+DHPG (G1+D) in the presence of MG132. Samples were then analyzed by Western blotting before (input) or after immunoprecipitation (IP) with GluA1 antibody or IgG. The right panel shows an immunoblot for GluA1 and a merged image of immunoblots for GluA1 (red) and ubiquitin (green). Asterisks indicate bands corresponding to ubiquitinated GluA1.

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