Figure 5. G1 induces GluA1... | Rockefeller University Press

$Figure 5. G1 induces GluA1 internalization via mTOR-dependent protein synthesis. (A–D) Whole hippocampal slices were pretreated with DMSO, cycloheximide, or rapamycin for 30 min and then incubated with G1 for an additional 60 min or the indicated times. CA1 and CA3-DG mini-slices were treated with G1 or E2 for 1 h. At the end of treatments, samples were homogenized and processed for Western blotting after membrane fractionation. Data are presented as the ratio (fold of control) of GluA1 over actin (A; n = 4–8), GluA2/3 over actin (B; n = 4–7), PSD95 over actin (C; n = 4–8), or GluA1 over actin (D–F; n = 3–11). *, P < 0.05; **, P < 0.01; ***, P < 0.001, as compared with control. #, P < 0.05; ###, P < 0.001, as compared with G1 alone (one- or two-way ANOVA). The actin panels in both A and C look very similar because they show the same data. Images in these two panels come from the same blot, in which different primary and secondary antibodies were used. Thus, the same actin blot serves as loading control for both GluA1 and PSD95. They have been separated in two panels for consistency in figure formatting. Error bars indicate means ± SEM.$

Figure 5.

G1 induces GluA1 internalization via mTOR-dependent protein synthesis. (A–D) Whole hippocampal slices were pretreated with DMSO, cycloheximide, or rapamycin for 30 min and then incubated with G1 for an additional 60 min or the indicated times. CA1 and CA3-DG mini-slices were treated with G1 or E2 for 1 h. At the end of treatments, samples were homogenized and processed for Western blotting after membrane fractionation. Data are presented as the ratio (fold of control) of GluA1 over actin (A; n = 4–8), GluA2/3 over actin (B; n = 4–7), PSD95 over actin (C; n = 4–8), or GluA1 over actin (D–F; n = 3–11). *, P < 0.05; **, P < 0.01; ***, P < 0.001, as compared with control. #, P < 0.05; ###, P < 0.001, as compared with G1 alone (one- or two-way ANOVA). The actin panels in both A and C look very similar because they show the same data. Images in these two panels come from the same blot, in which different primary and secondary antibodies were used. Thus, the same actin blot serves as loading control for both GluA1 and PSD95. They have been separated in two panels for consistency in figure formatting. Error bars indicate means ± SEM.

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