Figure 3. Estrogen stimulates mTOR-dependent protein synthesis via GPER1 activation. Hippocampal slices were pretreated with DMSO, G36, cycloheximide, or rapamycin for 30 min and then incubated with G1 or E2 for an additional 30–60 min. Treatments with PPT (100 nM) or DPN (10 nM) were for 1 h. At the end of treatments, samples were homogenized and processed for Western blotting before (A) or after (B–D) membrane fractionation. Data are presented as the ratio (fold of control) of αCaMKII over actin (A; n = 5–12) or Arc over actin (B–D; n = 3–14). *, P < 0.05; **, P < 0.01, as compared with control. #, P < 0.05 as compared with E2 alone (one- or two-way ANOVA). Black lines indicate that intervening lanes have been spliced out. Error bars indicate means ± SEM.
Figure 3.

Estrogen stimulates mTOR-dependent protein synthesis via GPER1 activation. Hippocampal slices were pretreated with DMSO, G36, cycloheximide, or rapamycin for 30 min and then incubated with G1 or E2 for an additional 30–60 min. Treatments with PPT (100 nM) or DPN (10 nM) were for 1 h. At the end of treatments, samples were homogenized and processed for Western blotting before (A) or after (B–D) membrane fractionation. Data are presented as the ratio (fold of control) of αCaMKII over actin (A; n = 5–12) or Arc over actin (B–D; n = 3–14). *, P < 0.05; **, P < 0.01, as compared with control. #, P < 0.05 as compared with E2 alone (one- or two-way ANOVA). Black lines indicate that intervening lanes have been spliced out. Error bars indicate means ± SEM.

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