TRAPPII is required for male fertility, and TRAPPIII is essential for viability. (A) Summary of mutations generated in TRAPP subunits in this and previous studies. The nature of the lesions in the EMS alleles of TRAPPC2, TRAPPC8, and TRAPPC11 were determined by sequencing the locus from the relevant mutants (Fig. S2 B). (B) Mass spectrometry analysis of TRAPPC9–SBP-GFP tandem affinity–purified from a WT or TRAPPC10[16] mutant background. Total spectral counts for each protein are shown. Subunits with a thick line in the cartoon are essential for Rab1 nucleotide exchange activity in yeast. (C) TRAPPC11 is essential for adult (blue bars) and pupal (green bars) viability. The bars show mean numbers of adult or pupal progenies calculated from two parallel crosses (pupal lethality of TRAPPC11[MB06920]/deficiency heterozygotes and TRAPPC11[63BD2]/deficiency heterozygotes) or three parallel crosses (all other heterozygotes). The mean value is shown above each bar. Note that TRAPPC11[63BD2]/deficiency heterozygotes occasionally develop into pupae, suggesting the allele is a strong hypomorph. (D) TRAPPC9 and TRAPPC10 are not essential for adult viability. The bars show the mean number of adult progeny obtained in three independent crosses. (E) TRAPP9 and TRAPPC10 are required for male fertility. The bars show the mean number of pupal progeny from three independent crosses using males with the indicated allele over the relevant deficiency. Error bars show SD.