Figure 9. KKT4 is dispensable for the localization of many kinetochore proteins. (A) Kinetochore localization of YFP-tagged KKT1, KKT2, KKT3, KKT7, KKT14, and KKIP1 is not affected by KKT4 knockdown. Controls are uninduced cell cultures. Example of anaphase cells fixed 48 h postinduction are shown (n > 20 anaphase cells; BAP1236, BAP1237, BAP1238, BAP1240, BAP1242, and BAP1243). (B) Kinetochore localization of YFP-KKT10 is not disrupted by KKT4 knockdown (n > 20 cells in 2K1N; BAP1241). (C) The KKT20 short isoform (Tb927.8.4760.1:mRNA) localizes at kinetochores throughout the cell cycle in unperturbed cells (BAP1244). Note that the Tb927.8.4760.2:mRNA long isoform has less bright signal and localizes at kinetochores from S phase to anaphase (Nerusheva and Akiyoshi, 2016). (D) Kinetochore localization of the KKT20 short isoform is significantly diminished in >80% of cells upon KKT4 knockdown (n > 30 cells each in the 1K1N, 2K1N, and 2K2N category). Bars, 2 µm.
Figure 9.

KKT4 is dispensable for the localization of many kinetochore proteins. (A) Kinetochore localization of YFP-tagged KKT1, KKT2, KKT3, KKT7, KKT14, and KKIP1 is not affected by KKT4 knockdown. Controls are uninduced cell cultures. Example of anaphase cells fixed 48 h postinduction are shown (n > 20 anaphase cells; BAP1236, BAP1237, BAP1238, BAP1240, BAP1242, and BAP1243). (B) Kinetochore localization of YFP-KKT10 is not disrupted by KKT4 knockdown (n > 20 cells in 2K1N; BAP1241). (C) The KKT20 short isoform (Tb927.8.4760.1:mRNA) localizes at kinetochores throughout the cell cycle in unperturbed cells (BAP1244). Note that the Tb927.8.4760.2:mRNA long isoform has less bright signal and localizes at kinetochores from S phase to anaphase (Nerusheva and Akiyoshi, 2016). (D) Kinetochore localization of the KKT20 short isoform is significantly diminished in >80% of cells upon KKT4 knockdown (n > 30 cells each in the 1K1N, 2K1N, and 2K2N category). Bars, 2 µm.

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