KKT4 is an inner kinetochore protein. (A) tdTomato-KKT4 colocalizes with YFP-KKT2 in metaphase (n > 25 cells) and early anaphase cells (n = 8 cells; BAP1272). Linescans for the area indicated by white lines (1.5 µm) are shown on the right. (B) YFP-KKIP1 appears as pairs of dots in metaphase cells, while tdTomato-KKT4 appears as individual dots (n = 25 cells). In early anaphase, KKIP1 is closer to spindle poles than KKT4 (n = 14 cells; BAP1273).