Figure 7. KKT4Δ115–174 fails to support proper chromosome segregation. (A) Expression of wild-type KKT4-YFP, but not KKT4Δ115–174-YFP, rescues the KKT4 3′ UTR–targeting RNAi phenotype. Controls are uninduced cell cultures. Error bars represent SD from three experiments (BAP1450 and BAP1484). (B) Examples of cells expressing KKT4-YFP (wild type or Δ115–174) fixed at 30 h postinduction and showing a number of lagging kinetochores in anaphase cells expressing KKT4Δ115–174. Bar, 2 µm. (C) Quantification of anaphase cells with lagging kinetochores at 30 h postinduction showing that RNAi-induced cells expressing KKT4Δ115–174 have significantly more lagging kinetochores than uninduced control (**, P < 0.0001, Fisher’s exact test) or RNAi-induced cells expressing wild-type KKT4 (*, P < 0.001, Fisher’s exact test; n > 300 anaphase cells for each).
Figure 7.

KKT4Δ115–174 fails to support proper chromosome segregation. (A) Expression of wild-type KKT4-YFP, but not KKT4Δ115–174-YFP, rescues the KKT4 3′ UTR–targeting RNAi phenotype. Controls are uninduced cell cultures. Error bars represent SD from three experiments (BAP1450 and BAP1484). (B) Examples of cells expressing KKT4-YFP (wild type or Δ115–174) fixed at 30 h postinduction and showing a number of lagging kinetochores in anaphase cells expressing KKT4Δ115–174. Bar, 2 µm. (C) Quantification of anaphase cells with lagging kinetochores at 30 h postinduction showing that RNAi-induced cells expressing KKT4Δ115–174 have significantly more lagging kinetochores than uninduced control (**, P < 0.0001, Fisher’s exact test) or RNAi-induced cells expressing wild-type KKT4 (*, P < 0.001, Fisher’s exact test; n > 300 anaphase cells for each).

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