Figure 6. KKT4 is essential for accurate chromosome segregation. (A) RNAi-mediated knockdown of KKT4 leads to cell growth defects. Control is uninduced cell culture. Cultures were diluted at 48 h. Error bars represent SD from six experiments (BAP1082). Similar results were obtained using two independent RNAi constructs targeting different regions of the KKT4 transcript. (B) Cells expressing YFP-KKT4 and tdTomato-KKT2 were fixed at 48 h after induction, showing a number of lagging kinetochores in anaphase. Note that YFP-KKT4 signal was reduced by RNAi. Bar, 2 µm. (C) Quantification of anaphase cells with lagging kinetochores at 48 h postinduction showing that RNAi-induced cells have significantly more lagging kinetochores than uninduced control (*, P < 0.0001, Fisher’s exact test, n > 300 anaphase cells).
Figure 6.

KKT4 is essential for accurate chromosome segregation. (A) RNAi-mediated knockdown of KKT4 leads to cell growth defects. Control is uninduced cell culture. Cultures were diluted at 48 h. Error bars represent SD from six experiments (BAP1082). Similar results were obtained using two independent RNAi constructs targeting different regions of the KKT4 transcript. (B) Cells expressing YFP-KKT4 and tdTomato-KKT2 were fixed at 48 h after induction, showing a number of lagging kinetochores in anaphase. Note that YFP-KKT4 signal was reduced by RNAi. Bar, 2 µm. (C) Quantification of anaphase cells with lagging kinetochores at 48 h postinduction showing that RNAi-induced cells have significantly more lagging kinetochores than uninduced control (*, P < 0.0001, Fisher’s exact test, n > 300 anaphase cells).

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