Figure 3. KKT4115–343 is sufficient for microtubule binding. (A) Schematic of the KKT4 protein sequence. Several features conserved among kinetoplastids are shown, with the truncated proteins used in Fig. 3 (B and C) indicated below. (B) Microtubule sedimentation assays of KKT4 fragments showing that KKT4115–343 can bind microtubules, whereas KKT42–114, KKT4250–472, and KKT4462–645 do not. P and S stand for pellet and supernatant, respectively. Dots indicate KKT4 fragments tested in the assay. (C) Microtubule sedimentation assays of KKT4 fragments showing that KKT4115–174 can bind microtubules, albeit to a lesser extent than KKT4115–343. (D) The charge-reversal mutant KKT4 has reduced microtubule-binding activity.
Figure 3.

KKT4115–343 is sufficient for microtubule binding. (A) Schematic of the KKT4 protein sequence. Several features conserved among kinetoplastids are shown, with the truncated proteins used in Fig. 3 (B and C) indicated below. (B) Microtubule sedimentation assays of KKT4 fragments showing that KKT4115–343 can bind microtubules, whereas KKT42–114, KKT4250–472, and KKT4462–645 do not. P and S stand for pellet and supernatant, respectively. Dots indicate KKT4 fragments tested in the assay. (C) Microtubule sedimentation assays of KKT4 fragments showing that KKT4115–174 can bind microtubules, albeit to a lesser extent than KKT4115–343. (D) The charge-reversal mutant KKT4 has reduced microtubule-binding activity.

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