Figure 2. Conserved subcellular... | Rockefeller University Press

Figure 2.

$Figure 2. Conserved subcellular localizations at the NE and in the cytoplasm. (A) TMEM258 is membrane associated. Western blot (WB) of soluble (Sol) and membrane (Mem) fractions of TMEM258-Myc–expressing 293T cells probed with anti-GAPDH (cytoplasm), anti-Calnexin (ER and ONM), anti-Emerin (INM), and anti-Myc antibodies. (B–C′′) The overexpressed TMEM258-Myc in 293T cells (B) and the endogenous Kud in ovarian follicle cells (C) locate at the cytoplasm and colocalize with NE marker lamins (B′ and C′, arrows). Merges are shown in B′′ and C′′. Bars, 10 µm.$

Conserved subcellular localizations at the NE and in the cytoplasm. (A) TMEM258 is membrane associated. Western blot (WB) of soluble (Sol) and membrane (Mem) fractions of TMEM258-Myc–expressing 293T cells probed with anti-GAPDH (cytoplasm), anti-Calnexin (ER and ONM), anti-Emerin (INM), and anti-Myc antibodies. (B–C′′) The overexpressed TMEM258-Myc in 293T cells (B) and the endogenous Kud in ovarian follicle cells (C) locate at the cytoplasm and colocalize with NE marker lamins (B′ and C′, arrows). Merges are shown in B′′ and C′′. Bars, 10 µm.