![Figure 6. Ablation of Sam68 ameliorates innervation of NMJs. (A) NMJ immunofluorescence images from whole-mount FDB2 of 10-dpp non-SMA, SMAΔ7/Sam68+/+, and SMAΔ7/Sam68−/− (SMA) mice. Higher magnifications are shown in the bottom panels. Postsynaptic (AChRs stained with α-bungarotoxin; green), presynaptic (stained with α-synaptophysin; red), and motor neuron axons (stained with an antibody against the heavy chain of neurofilament; red) were visualized. Bars, 100 µm. Arrows point to innervated NMJs (yellow staining), and arrowheads point to denervated NMJs (green staining). (B) Bar graph showing the percentage of innervated (left) and denervated (right) NMJs in FDB2 of non-SMA, non-SMA/Sam68−/−, SMAΔ7/Sam68+/+, and SMAΔ7/Sam68−/− mice (for all, n = 3). Quantification of NMJ innervation was performed on at least 100 optical sections for mice, with a step size of 3 µm from the whole FDB2 of each genotype. Data represent mean ± SEM. Statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparison posttest (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant [P > 0.05]).](https://cdn.rupress.org/rup/content_public/journal/jcb/211/1/10.1083_jcb.201502059/6/jcb_201502059_fig6.jpeg?Expires=1773553963&Signature=4bVUFYPEv8MfknOsO3rRIArVSauo2pnOHJo406sAjCMUFn2Ytcgrtn1DGqFhaG~GSdRB6tqlG~uOt6RtK9xYNgOoBRrvj0ixqWp5xUZ6SsO8xlQHNWu-xVPWCXxaqIiYojg8E4zmYaydz88Q-lLOgz~q-za~YHQIVHgFo72XG1Qxtq618uVN7PUastwoN3un7DccnCSSmmdRHbscvdsbtGUrtpnHT6goPSIRfKxTCUNGK5DRTHXmZnwoFKL89Wey43ilpNU0KZS4gMvMRbtBpByOncOXhyWhvt~kv5Hg19SE5TeUGTOD1d2yVI-1wT93BoTJl313HEPW-7rEUNohLQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ablation of Sam68 ameliorates innervation of NMJs. (A) NMJ immunofluorescence images from whole-mount FDB2 of 10-dpp non-SMA, SMAΔ7/Sam68+/+, and SMAΔ7/Sam68−/− (SMA) mice. Higher magnifications are shown in the bottom panels. Postsynaptic (AChRs stained with α-bungarotoxin; green), presynaptic (stained with α-synaptophysin; red), and motor neuron axons (stained with an antibody against the heavy chain of neurofilament; red) were visualized. Bars, 100 µm. Arrows point to innervated NMJs (yellow staining), and arrowheads point to denervated NMJs (green staining). (B) Bar graph showing the percentage of innervated (left) and denervated (right) NMJs in FDB2 of non-SMA, non-SMA/Sam68−/−, SMAΔ7/Sam68+/+, and SMAΔ7/Sam68−/− mice (for all, n = 3). Quantification of NMJ innervation was performed on at least 100 optical sections for mice, with a step size of 3 µm from the whole FDB2 of each genotype. Data represent mean ± SEM. Statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparison posttest (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant [P > 0.05]).
