Sam68 deletion rescues SMN2 splicing and expression in SMAΔ7 tissues. (A) qPCR analysis of exon 7–containing SMN2 transgene transcripts normalized to constant exon 6 in tissues of 10-dpp SMAΔ7/Sam68^+/+ and SMAΔ7/Sam68^−/− mice. Bar graph represents mean ± SD. n = 3. The p-value was determined by two-tailed t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). (B) Western blot analysis of SMN protein expression in the indicated tissues of 10-dpp SMAΔ7/Sam68^+/+ (wt) or SMAΔ7/Sam68^−/− (ko) mice. Actin was used as a loading control. Quantification of the SMN band is shown at the bottom. Each point value represents the mean ± SD. n = 2. Cereb, cerebellum; ko, knockout; S.cord, spinal cord; wt, wild type.