Increased Ccl5 and Cxcl10 secretion by FIP200-deficient NSCs enhanced microglia migration. (A and B) Number of migrated microglia using naive media or conditioned media from neurospheres of Ctrl and FIP cKO mice (A) or 2cKO and p53 cKO mice (B) as attractant (mean ± SEM; six mice each). (C and D) Number of microglia cultured with naive media or conditioned media from neurospheres of Ctrl and FIP cKO mice (C) or 2cKO and p53 cKO mice (D; mean ± SEM; six mice each). (E) Normalized number of microglia migrating toward various conditioned media (mean ± SEM). Conditioned media are from neurospheres of Ctrl, FIP cKO, 2cKO, and p53 cKO mice that had been infected with recombinant lentiviruses encoding Ctrl shRNA, Ccl5 shRNA, Cxcl10 shRNA, or a combination of Ccl5 and Cxcl10 shRNAs, as indicated (three mice each). (F–H) Number of microglia migrating toward conditioned media from neurospheres of FIP cKO (F), 2cKO (G), and p53 cKO (H) mice (mean ± SEM). Conditioned media was treated with an unrelated antibody, Ccl5 antibody, Cxcl10 antibody, or a combination of Ccl5 and Cxcl10 antibodies at different concentrations (three mice each). (I) Number of microglia migrating toward conditioned media from neurospheres of Ctrl, FIP cKO, 2cKO, and p53 cKO mice (mean ± SEM). Conditioned media was treated with DMSO vehicle (Veh), 1 µM AMG-487 (AMG), 1 µM maraviroc (MAR), or 10 µM TAK-779 (TAK; three mice each). CM, conditioned media. *, P < 0.05; **, P < 0.01; ***, P < 0.001.