p53-independent increase in Ccl5 and Cxcl10 expression in FIP200-deficient NSCs. (A) Venn diagram showing the overlap between genes with at least twofold increase or decrease compared with Ctrl mice in neurospheres derived from FIP cKO and 2cKO mice, but not those from p53 cKO mice (yellow) and those with similar changes in all samples (red). The number of genes is indicated. (B–F) Quantitative RT-PCR of Ccl5 (B), Cxcl10 (C), Ifit1 (D), Ifit3 (E), and Ifi44 (F) in neurospheres from Ctrl, FIP cKO, 2cKO, and p53 cKO mice (four mice each). (G) Lysates were extracted from SVZ of Ctrl, FIP cKO, 2cKO, and p53 cKO mice (three mice each) at P28 and analyzed by immunoblot using antibodies to detect FIP200, Ccl5, Cxcl10, and actin. (H–K) Immunofluorescence of Ccl5 (H and J) and Cxcl10 (I and K) with DAPI in the SVZ (H and I) and neurospheres (J and K; three mice each) of Ctrl, FIP cKO, 2cKO, and p53 cKO mice at P28. (L and M) Concentration of Ccl5 (L) and Cxcl10 (M) in conditioned neurosphere media (normalized to 1 mg total protein lysate of neurospheres) in Ctrl, FIP cKO, 2cKO, and p53 cKO mice (mean ± SEM; nine mice each). LV, lateral ventricle; ST:, striatum; SVZ, subventricular zone. Bars: (H and I) 50 µm; (J and K) 20 µm. *, P < 0.05; **, P < 0.01; ***, P < 0.001, NS, not significant; RFI, relative fluorescence intensity.