Increased infiltration of microglia in FIP200-deficient SVZ. (A) Immunofluorescence of Iba1 and DAPI in the SVZ (three mice each) in Ctrl, FIP cKO, 2cKO and p53 cKO mice at P28. Arrows indicate Iba1⁺ microglia. Bar, 40 µm. (B and C) Number of Iba1⁺ cells per SVZ (B) and RMS (C) section in Ctrl and FIP cKO mice at P0, P7, P14, and P28 (mean ± SEM; six mice per time point). (D) Percentage of Ki67⁺ microglia (Iba1⁺) per SVZ section in Ctrl and FIP cKO mice at P0, P7, P14, and P28 (mean ± SEM; >600 Iba1⁺ cells from six mice per time point). (E) Lysates were extracted from in vitro cultured astrocytes and microglia treated with DMSO or BafA1 for 1 h and analyzed by immunoblot to detect FIP200, LC3, p62, and actin. (F and G) Number of Iba1⁺ cells per SVZ (F) and RMS (G) section in Ctrl, FIP cKO, 2cKO, and p53 cKO mice at P28 (mean ± SEM; six mice each). (H) Percentage of Ki67⁺ microglia (Iba1⁺) per SVZ section of Ctrl, FIP cKO, 2cKO, and p53 cKO mice at P28 (mean ± SEM; >600 Iba1⁺ cells from six mice each). E, ependymal layer; LV, lateral ventricle; RMS, rostral migratory stream; ST, striatum; SVZ, subventricular zone. *, P < 0.05; **, P < 0.01; ***, P < 0.001.