DUB activity of BRISC contributes to the proper mitotic spindle assembly. (A) Representative live-cell still images of H2B-GFP/α-tubulin-mRFP HeLa cells transfected with control (see Video 5) or BRCC36 siRNA (see Video 6), or cotransfected with BRCC36 siRNA and CFP-BRCC36res WT (see Video 7) or BRCC36 siRNA and CFP-BRCC36res QSQ, without DUB activity (see Video 8). NEBD indicates the first frame after NEBD, based on the chromatin marker H2B-GFP. Times are shown in hours:minutes. (B) Quantification of the cells with bipolar or multipolar spindles as shown in A, calculated as described in Fig. 2 C. (C) Quantification of the cells with aligned or misaligned chromosomes as shown in A, calculated as described in Fig. 2 D. (D) Box-and-whisker plots showing the duration of the cells, shown in Fig. 5 A, spent in mitosis from NEBD to metaphase and metaphase to anaphase onset or cell death. *, P < 0.05; ***, P < 0.001; Student’s t test. ns, no significance. Error bars show mean ± SD.