Figure 4. Diffusion coefficients of 488neg-SM, 594neg-SM, 594neg-DSPC, and 594neg-DOPC in Lo and Ld domains in Lo-Ld phase–separated GUVs, determined by FCS. (A) Autocorrelation functions G(τ) values for the time-dependent changes of the signal intensities of the fluorescent probes observed at typical diffraction-limited areas (white spots) shown in the image on the right in Lo and Ld domains (the spots in the images are expanded 5× to show their locations clearly). The black solid smooth curves indicate the best-fit functions, providing the diffusion coefficients (D). Bars, 10 µm. 594neg-DSPC was examined in both Lo-Ld phase–separated GUVs consisting of SM(18:0)/DOPC/cholesterol and those made of DSPC/DOPC/cholesterol (1:1:1 by moles). (B) Mean ± SE values of D in the Ld and Lo domains (n = 24, 24, 31, 27, 24, 31, 25, 24, 25, and 25 GUVs from top to bottom). See also Figs. 2 and 3.
Figure 4.

Diffusion coefficients of 488neg-SM, 594neg-SM, 594neg-DSPC, and 594neg-DOPC in Lo and Ld domains in Lo-Ld phase–separated GUVs, determined by FCS. (A) Autocorrelation functions G(τ) values for the time-dependent changes of the signal intensities of the fluorescent probes observed at typical diffraction-limited areas (white spots) shown in the image on the right in Lo and Ld domains (the spots in the images are expanded 5× to show their locations clearly). The black solid smooth curves indicate the best-fit functions, providing the diffusion coefficients (D). Bars, 10 µm. 594neg-DSPC was examined in both Lo-Ld phase–separated GUVs consisting of SM(18:0)/DOPC/cholesterol and those made of DSPC/DOPC/cholesterol (1:1:1 by moles). (B) Mean ± SE values of D in the Ld and Lo domains (n = 24, 24, 31, 27, 24, 31, 25, 24, 25, and 25 GUVs from top to bottom). See also Figs. 2 and 3.

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