The myosin-V Myo2p regulates the time of secretory vesicle tethering. (A) Kymograph of GFP-Sec4 vesicle puncta shown in a myo2-13 strain at the permissive temperature. (B) Kymograph of GFP-Sec4 vesicle puncta shown in a myo2-3DR strain. (C) Kymograph of GFP-Sec4 vesicle puncta shown in a myo2-RAKA strain. (D) Column scatter plot of the time from tethering to fusion (in seconds) in wild-type (n = 22), myo2-13 (n = 15), myo2-3DR (n = 14), and myo2-RAKA (n = 16) cells at 21°C. ***, P < 0.0001; ns, not significant. Lines show mean and 95% confidence intervals.