Smaller oocytes spend longer in prometaphase but then have a faster rate of securin loss. (A) Relative securin degradation traces from B3 cytoplasts lacking chromosomes after bisection. (B) Representative time lapse of B0 (top) and B3 (bottom) oocytes expressing securin-YFP and H2B-mCherry relative to time after NEB. Bar, 10 µm. (C) Timing of anaphase relative to NEB, determined from images as in B. There is no significant difference in the time of anaphase between B0 and B3 oocytes. (D) Timing of NEB (left) and anaphase (right) relative to activation of the APC. Plots for individual oocytes are displayed above, with means below. (E) Individual (pale) and mean (bold) securin traces from B0 and B3 oocytes, arranged relative to the time of APC activation. Horizontal bars show the corresponding timing of anaphase. (F) Maximal rate of securin destruction recorded from traces as in E. In C, D, and F, numbers of oocytes used are indicated in parentheses. In C, D, and F, statistical test was unpaired t test. Error bars indicate SD.
