FOCAL POINT  Kirk Donovan (left) and Tony Bretscher (right) track the behavior of individual secretory vesicles in budding yeast in order to provide a timeline of the events involved in exocytosis. As shown in this time-lapse series, vesicles labeled with the Rab GTPase Sec4p consistently tether to the bud cortex for 18 seconds (red arrowheads) before undergoing membrane fusion and disappearing (final frame). This tethering period, which may allow cells to ensure vesicles are targeted to the right location, is regulated by Sec4p and the myosin motor Myo2p. Myo2p dissociates from the vesicles about four seconds before fusion, in contrast to other components, such as the exocyst complex, that remain bound to vesicles until exocytosis is completed. / PHOTOS COURTESY OF THE AUTHORS

FOCAL POINT  Kirk Donovan (left) and Tony Bretscher (right) track the behavior of individual secretory vesicles in budding yeast in order to provide a timeline of the events involved in exocytosis. As shown in this time-lapse series, vesicles labeled with the Rab GTPase Sec4p consistently tether to the bud cortex for 18 seconds (red arrowheads) before undergoing membrane fusion and disappearing (final frame). This tethering period, which may allow cells to ensure vesicles are targeted to the right location, is regulated by Sec4p and the myosin motor Myo2p. Myo2p dissociates from the vesicles about four seconds before fusion, in contrast to other components, such as the exocyst complex, that remain bound to vesicles until exocytosis is completed.

PHOTOS COURTESY OF THE AUTHORS

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