Plant ferroptosis involves GSH and AA oxidation and depletion. (a) AA levels in A. thaliana roots before or after treatment at 55°C or 77°C with or without the ferroptosis inhibitors Fer-1 and CPX. FW, fresh weight. (b) GSH levels in A. thaliana roots before (Ct., control) or after treatment at 55°C (55) or at 77°C with or without the ferroptosis inhibitors Fer-1 (F) and CPX (C). Data are the mean ± SEM of three independent experiments. Different letters denote statistical difference (one-way analysis of variance, P < 0.05). (c) 6-d-old seedlings were preincubated with 100 µM GSH or DMSO overnight (16 h). Cell death was induced by treating roots at 55°C. (d) Arabidopsis seedlings were grown in control plates or 10 µM l-buthionine-(S,R)-sulfoximine (BSO)–supplemented plates. 6-d-old seedlings were analyzed. (c and d) Root hairs were stained with Sytox green, and Sytox-positive (interpreted as dead cells) and Sytox-negative cells and the number of dead and living root hairs was quantified. Results are expressed as a percentage of dead cells. Data are mean ± SEM of three independent experiments. Different letters denote statistical difference (one-way analysis of variance, P < 0.05).