Lamellipodin and Arp2/3 are involved in lateral membrane motility. (A) Co-staining for lamellipodin (Lpd) and F-actin in EpH4 cells treated with control or DAAM1 siRNA. Lpd overlapped with F-actin at LCs. (B) Co-staining for Lpd and Abi1. (C) Immunostaining for E-cadherin in EpH4 cells treated with DAAM1 and/or Lpd siRNAs. Depletion of Lpd suppressed the diffused distribution of E-cadherin induced by DAAM1 KD. (D) Quantification of the tilting extent in C. (E) Immunostaining for E-cadherin in control or DAAM1-depleted EpH4 cells treated with 150 µM of the Arp2/3 inhibitor CK-666 in DMSO or DMSO only (0.15% in final concentration) for 30 min. Diffused distribution of E-cadherin induced by DAAM1 KD was partially suppressed. (A–C and E) Arrowheads point to the basal edges of the junctions. Bars, 10 µm. (F) Quantification of the tilting extent in E. (D and F) Histograms represent the mean of three experiments. Error bars indicate SD. **, P < 0.01; ***, P < 0.001; n.s., not significant.