Figure 9.
Figure 9. PIX-1/PAK-1 and LET-502 are antagonistic in dorsal and lateral anterior cells. Violin plot representing the distribution and mean AJM-1::GFP anisotropy measured at dorsal anterior D1 and D2 junctions (A) and lateral anterior L1 and L2 junctions (B) in wt, let-502(RNAi), pix-1(gk416), pix-1(gk416); let-502(RNAi), pak-1(gk416), and pak-1(gk416);let-502(RNAi) embryos expressing AJM-1::GFP and VAB-10(ABD)::mCherry as well as nmy-2(ne1490ts); ajm-1::GFP at 18°C or 25°C. (C) Density of AJM-1::GFP clusters measured for the lateral anterior L1 and L2 junctions. The dashed lines indicate the mean value for wt (black), pix-1(gk416) (blue), and let-502(RNAi) (green). (D) Schematic representation of cluster density and anisotropy for each strain. N > 10 embryos were analyzed per genotype and junction. t test p-values significantly different from wt (*) and let-502(RNAi) (#) are indicated in parentheses.

PIX-1/PAK-1 and LET-502 are antagonistic in dorsal and lateral anterior cells. Violin plot representing the distribution and mean AJM-1::GFP anisotropy measured at dorsal anterior D1 and D2 junctions (A) and lateral anterior L1 and L2 junctions (B) in wt, let-502(RNAi), pix-1(gk416), pix-1(gk416); let-502(RNAi), pak-1(gk416), and pak-1(gk416);let-502(RNAi) embryos expressing AJM-1::GFP and VAB-10(ABD)::mCherry as well as nmy-2(ne1490ts); ajm-1::GFP at 18°C or 25°C. (C) Density of AJM-1::GFP clusters measured for the lateral anterior L1 and L2 junctions. The dashed lines indicate the mean value for wt (black), pix-1(gk416) (blue), and let-502(RNAi) (green). (D) Schematic representation of cluster density and anisotropy for each strain. N > 10 embryos were analyzed per genotype and junction. t test p-values significantly different from wt (*) and let-502(RNAi) (#) are indicated in parentheses.

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