Hypodermal cells produce convergent protrusions during early elongation. Lamellipodia-like (A) and amoeboid-like (B) protrusions produced by dorsal and lateral cells, respectively, observed in embryos expressing AJM-1::GFP (green) and VAB-10(ABD)::mCherry (red). Locations of the enlarged images are indicated by a white box. z-projection (xy plane) (C) and zy plane (D) of an embryo expressing AJM-1::GFP (green) and Kaede (red) in hypodermal cells. Location of the enlarged images is indicated by a white box. The z-depth is represented by artificial color from the surface (0 µm; red) to 4-µm depth (green). The arrow and arrowhead indicate the cell body and the protrusion, respectively. (E) Time lapse recording of AJM-1::GFP (green) and Kaede (red). Bars: 10 µm; (enlarged images) 5 µm. (F) F-actin accumulation at the neck (arrow) and leading edge (LE; arrowhead) of amoeboid-like protrusion visualized in embryo expressing VAB-10(ABD)::mCherry and on the intensity plot along the dashed arrow indicated in the enlarged picture. (G) Myosin heavy-chain, NMY-2::GFP (green) accumulation observed at DLG-1/AJM-1 complex junction (J; arrow) in embryo expressing DLG-1::RFP (red) and on the intensity plot along the dashed arrow indicated in the enlarged picture. Cell body (CB) and the apical pole of amoeboid protrusion (Protr.) are indicated. Bars: 10 µm; (enlarged images) 2 µm. Data are representative of three independent experiments.