let-502and pix-1/pak-1 control the remodeling of the apical junctions between lateral and dorsal cells, respectively. Mean of AJM-1::GFP anisotropy measured for junction between dorsal (A), lateral (B), and ventral (C) anterior cells in wt, let-502(RNAi), pix-1(gk416), and pak-1(ok448) embryos expressing AJM-1::GFP and VAB-10(ABD)::mCherry. N > 10 embryos were analyzed per genotype and junction. Significant t test p-values are indicated in parentheses. *, t test p-value significantly different from wt; #, t test p-value significantly different between L1 and L2 junctions in wt. Error bars indicate SEM.