The ciliogenesis effector Cep164 affects microtubule stabilization and actomyosin contractility upon serum starvation. (A) Identification of cold-resistant microtubules in serum-starved and serum-fed cells treated with siRNA against Cep164. Images show four representative examples of Cep164-depleted serum-starved and Cep164-depleted serum-fed cells (experiments obtained with two distinct Cep164 siRNA sequences). Cells fixed after a brief cold shock of 12 min after 3 h of serum starvation. The graph shows measurements of α-tubulin fluorescence intensity after cold shock (LUT fire; results of two independent experiments, n = 110 cells per condition). (B) Same as in A with siRNA against IFT88 (results of two independent experiments, n = 110 cells per condition). (C) Averaging of phosphomyosin fluorescence intensity levels (LUT fire), obtained by stacking and averaging 50 images per condition. Cells were fixed 4 h after serum starvation. Averaged images showed that myosin phosphorylation increased in control and IFT88-depleted cells, but not in Cep164-depleted cells. Bars, 10 µm. *, P < 0.05; ****, P < 0.0001. a.u., arbitrary units.