Figure 4.
Figure 4. Fragmentation of mitochondria does not accelerate mitophagy. (A and B) WT (A) and Drp1 KO (B) cells stably expressing mito-Keima were transfected with control or MFN1/2 siRNAs and cultured under mitophagy conditions as in Fig. 2 A. Bars, 10 µm. (C) Mitophagy dots shown in A and B were calculated as in Fig. 2 C. Data are shown as the mean ± SD of three independent experiments. (D) Cells transfected with control and MFN1/2 siRNAs were lysed and analyzed by immunoblotting with anti-MFN1, anti-MFN2, anti-Drp1, and anti-actin antibodies.

Fragmentation of mitochondria does not accelerate mitophagy. (A and B) WT (A) and Drp1 KO (B) cells stably expressing mito-Keima were transfected with control or MFN1/2 siRNAs and cultured under mitophagy conditions as in Fig. 2 A. Bars, 10 µm. (C) Mitophagy dots shown in A and B were calculated as in Fig. 2 C. Data are shown as the mean ± SD of three independent experiments. (D) Cells transfected with control and MFN1/2 siRNAs were lysed and analyzed by immunoblotting with anti-MFN1, anti-MFN2, anti-Drp1, and anti-actin antibodies.

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