Figure 2. Fop1 stabilizes BBs to resist ciliary beating. (A) BB loss in FOP1 KD cells is elevated at increasing temperatures. Representative BB rows (α-centrin) in WT and FOP1 KD cells at 25°C, 30°C, and 37°C. (B) Increased media viscosity using PEO exacerbates the BB loss phenotype in FOP1 KD cells. Representative BB rows in WT and FOP1 KD cells in untreated or PEO media. (C) Inhibition of ciliary beating using NiCl2 prevents high-temperature–induced BB loss in FOP1 KD cells. Representative BB rows in WT and FOP1 KD cells in untreated or NiCl2-treated media. Mean ± SEM; n = 200 rows. *, P < 0.01. Bar, 1 µm.
Figure 2.

Fop1 stabilizes BBs to resist ciliary beating. (A) BB loss in FOP1 KD cells is elevated at increasing temperatures. Representative BB rows (α-centrin) in WT and FOP1 KD cells at 25°C, 30°C, and 37°C. (B) Increased media viscosity using PEO exacerbates the BB loss phenotype in FOP1 KD cells. Representative BB rows in WT and FOP1 KD cells in untreated or PEO media. (C) Inhibition of ciliary beating using NiCl2 prevents high-temperature–induced BB loss in FOP1 KD cells. Representative BB rows in WT and FOP1 KD cells in untreated or NiCl2-treated media. Mean ± SEM; n = 200 rows. *, P < 0.01. Bar, 1 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal