Figure 1. Fop1 is a BB stability protein. (A) Whole-cell BB immunofluorescence (α-centrin). FOP1 knockdown (KD) cells have fewer BBs compared with wild type (WT). The cell’s anterior is defined by the oral apparatus (arrow). Bar, 10 µm. (B) Schematic of a 10-µm segment of BBs along a ciliary row that represents the region used for BB frequency analysis. Bar, 1 µm. (C, top) FOP1 KD cells have fewer BBs than WT cells. BB loss is rescued by the reintroduction of FOP1. Representative fluorescence images of BB rows in WT, FOP1 KD, and FOP1 KD with FOP1 rescue at 30°C and 37°C in cell cycle arrested cells. (bottom) Quantification of BBs per 10 µm. Mean ± SEM; n = 300 rows; *, P < 0.01. (D and E) FOP1 KD causes constricted BBs with defective C tubules (arrows). Electron micrographs of longitudinal (D) and cross-sectional (E) views of FOP1 KD BBs at 37°C. n = 50 BBs for each orientation. Bars, 100 nm.
Figure 1.

Fop1 is a BB stability protein. (A) Whole-cell BB immunofluorescence (α-centrin). FOP1 knockdown (KD) cells have fewer BBs compared with wild type (WT). The cell’s anterior is defined by the oral apparatus (arrow). Bar, 10 µm. (B) Schematic of a 10-µm segment of BBs along a ciliary row that represents the region used for BB frequency analysis. Bar, 1 µm. (C, top) FOP1 KD cells have fewer BBs than WT cells. BB loss is rescued by the reintroduction of FOP1. Representative fluorescence images of BB rows in WT, FOP1 KD, and FOP1 KD with FOP1 rescue at 30°C and 37°C in cell cycle arrested cells. (bottom) Quantification of BBs per 10 µm. Mean ± SEM; n = 300 rows; *, P < 0.01. (D and E) FOP1 KD causes constricted BBs with defective C tubules (arrows). Electron micrographs of longitudinal (D) and cross-sectional (E) views of FOP1 KD BBs at 37°C. n = 50 BBs for each orientation. Bars, 100 nm.

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